TODAY: Special Seminar, Bo Huang (UCSF), 4:15 PM in AP 200

Jason Pelc jpelc at
Thu Jun 10 11:22:39 PDT 2010

Please join the Stanford Optical Society for the following seminar 
presented by Prof. Bo Huang of the University of California, San 
Francisco.  Refreshments will be served at 4:00 PM in the Applied 
Physics building lobby.

*/Special Seminar/*

* *

*Bo Huang*

*University of California, San Francisco*

* *

*STORM: Super-Resolution Light Microscopy with Twinkling Molecules*

*/ /*

*/Thursday, June 10, 4:15 PM, AP 200.  Refreshments at 4:00/*

*/Presented by the Stanford Optical Society/*

*/ /*


The ability of fluorescence microscopy to perform noninvasive imaging of 
live samples with molecular specificity has made it one of the most 
powerful imaging techniques to study cellular processes. However, the 
diffraction of light limits the spatial resolution of conventional 
fluorescence microscopy, leaving many biological structures too small to 
be observed in detail. To overcome this limit, we have developed the 
Stochastic Optical Reconstruction Microscopy (STORM) technique. It 
utilizes the photoswitching of fluorophores to isolate their spatially 
overlapped images, and single-molecule localization to reconstruct the 
sample structure with the position of labeled fluorescent probes. We 
have achieved a 20-30 nm lateral resolution in cellular samples, which 
is an improvement by more than an order of magnitude over conventional 
fluorescence microscopy. The incorporation of three-dimensional (3D) 
single molecule localization further enables 3D STORM of a whole cell 
with 50-60 nm axial resolution. We have also created photoswitchable 
fluorophores for multicolor imaging by combinatorial pairing of various 
activator dyes and reporter dyes. We have demonstrated the ability of 
STORM to visualize structures unresolvable by conventional fluorescence 
microscopy, including in vitro reconstituted clathrin-mediated endocytic 
machinery and synapses in the olfactory system.

/About our speaker/

Tim Day PhotoDr. Bo Huang received his B.S. degree in Chemistry from 
Peking University in China in 2001. In 2006, he earned his Ph. D. degree 
in Chemistry at Stanford University under the direction of Dr. Richard 
N. Zare. After working as a postdoc in Dr. Xiaowei Zhuang's lab at 
Harvard University, he joined the faculty of the University of 
California, San Francisco in 2009 as an Assistant Professor of 
Pharmaceutical Chemistry and Biochemistry&  Biophysics. Dr. Huang's 
research work encompasses the area of bioanalysis, single molecule 
biophysics and optical microscopy. As a postdoc, he and his colleagues 
developed the super-resolution microscopy technique of STORM. He is 
currently interested in using optical methods to probe biological 
processes at the molecular scale. The awards that Dr. Huang has received 
include the Stanford Graduate Fellowship, the GE Healthcare and Science 
Prize for Young Life Scientists, and the Searle Scholar.

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