Special Seminar - Dr. Peter Kiesel (Palo Alto Research Center), Thursday April 05, 4:15PM, CISX 101
cachang at stanford.edu
Wed Mar 21 16:38:14 PDT 2012
Special Seminar Presented by the Stanford Optical Society
Opto-fluidic Detection System Enabling Sophisticated Point-of-care Diagnostics
Dr. Peter Kiesel
PARC (Palo Alto Research Center, Inc.)
Thursday, April 05, 4:15 PM, CISX 101 Auditorium
Refreshments at 4PM
The strategic landscape for biological and biomedical testing is undergoing a truly disruptive transformation. Today the majority of tests are performed at major, centralized clinical laboratories since compact, robust, and inexpensive instruments for point of care (POC) testing are not available. The principal drivers for POC testing are reducing costs, obtaining timely test results, lowering mortality rates, and reducing morbidity. We have demonstrated and prototyped a new optical detection approach that delivers high signal-to-noise discrimination – without complex optics, expensive detectors or bulky excitation sources. It therefore enables a truly compact and low-cost microfluidic-based instrument that can be used for diagnostics on whole blood or other complex fluids. The enabling technique is termed “spatially modulated emission” and generates a time-dependent signal as a continuously fluorescing bio-particle traverses a predefined pattern for optical transmission. Correlating the detected signal with the known pattern achieves high discrimination of the particle signal from background noise. The detection technique has been evaluated with measurements of CD4+ lymphocytes in human blood, which is required for initiation and monitoring the treatment of HIV-infected patients. T he technique has been benchmarked against a commercial instrument and excellent agreement for both absolute CD4 and percentage CD4 has been demonstrated. More recent experiments showed that our detection platform can address a large variety of diagnostic needs including multiplexed bead-based assays (ELISA on-the-flow) and identification and enumeration of pathogens (e.g., Giardia , Cryptosporidium and E.Coli ) in fluids.
About the speaker
Dr. Peter Kiesel , Principal Scientist, Palo Alto Research Center
Dr. Kiesel is conducting research in the areas of compact optical sensing systems, ultra sensitive light detection, and nitride based light emitters. Leading the optical detection group at PARC, Peter's current research and development activities include compact on-chip optical detection systems targeting bio-technological and medical applications. Key technologies that he has developed over the last 5 years include:
· Micro-fluidic-based optical detection platform for on-the-flow analyte characterization;
· Spatially modulated excitation and emission technique for analyte detection with improved signal-to-noise discrimination which enables point-of-care flow cytometers;
· Low-cost interrogation unit for wavelength-encoded optical sensors;
· Improved light/target interaction by guiding light in the fluid containing the analyte;
· Cavity-enhanced sensing, a method enabling on-the-flow absorption and refractive index measurements in a microfluidic device;
· Chip-size spectrometer which enables fluorescence spectroscopy on a chip; and
· Detection of individual bacteria based on native fluorescence. Dr. Kiesel is author or coauthor of more than 240 scientific publications including 90 refereed journal articles, 53 issued patents, 22 patent applications and 3 book chapters. He has organized many international workshops and conferences and has been the principal investigator on more than 12 research projects covering a large variety of sensing systems and optoelectronic devices (e.g., pathogen detection in water, micro-fluidic flow cytometer, bio-detection based on native fluorescence spectroscopy, highly efficient light emitters, light modulators, sensitive photo detectors, opto-optical switches, and polarization coded logic elements).
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