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Cleaning of Labware

Cleaning and decontamination procedure for new or dedicated labware.

Cleaning or Decontamination of Labware

 

Purpose:  This module covers the cleaning of new Teflon labware (cassettes, handles, polypropylene boxes, etc) and the decontamination of existing labware, for example, after a cassette has fallen on the floor or a handle has fallen into the plenum of a clean wet bench.  This procedure brings the labware to a clean (wbdiff, wbnonmetal) or semi-clean (wbsilicide).

 

This document covers the case of labware used in contaminated benches.  The cleaning procedure is the same but performed in a contaminated wetbench (wbgen or wbgaas).

 

The case of a full decontamination of a wet bench is described in a separate processing module.

 

Equipment: Wbsilicide or wbnitride depending on the size and shape of the labware for clean or semi-clean labware.  For contaminated labware use wbgen or wbgaas.

 

Time of Execution:  Approximately 60 minutes.

 

Step Summary:

 

Step 1:

Time =  30:00 minutes

Temperature = room temp

Chemical = 10 minutes in 5:1:1 H2O: H2O2: NH4OH (water: hydrogen peroxide: ammonium hydroxide:) to remove organics.

Rinse = dump rinser or overflow rinse

 

Step 2:

Time =  30:00 minutes

Temperature = room temp

Chemical = 10 minutes in 5:1:1 H2O: H2O2: HCl (water: hydrogen peroxide: hydrochloric acid) to remove trace metals.

Rinse = dump rinser or overflow rinse

Dry = air dry or air gun dry

 

Detailed Procedure:  Depending on the size of the labware to be cleaned you may choose to use wbsilicide or wbnitride.  Both procedures will be described.

For standard 4 and 6 inch Teflon cassettes, handles or Teflon baskets with long handles to be used at a clean wetbench use the wbsilicide:

  1. Use the clean hot pot (second hot pot to left hand side, verify with staff, this might change).  Dump the existing solution and rinse.  Refill with 5:1:1 H2O: H2O2: NH4OH.
  2. Place the labware into the hot pot. 
  3. Leave in the bath for 10 minutes at room temp.
  4. Dump rinse.
  5. Dump the solution, rinse well and refill with 5:1:1 H2O: H2O2: HCl.
  6. Place the labware into the hot pot.
  7. Leave in the bath for 10 minutes at room temp.
  8. Dump rinse.
  9. Air dry on a clean wipe or spin dry in the spin rinse dryer (for cassettes) or use the  N2 blow gun to dry.
  10. Dump the solution in the hot pot and rinse by filling and dumping the bath with DI water three times.

 

For smaller personal use labware to be used at a clean wetbench use the Tweezers cleaning beakers at the wbsilicide or a clean quartz beaker at wbgen or wbgaas:

  1. Fill one of the tweezer cleaning beakers with the solution of 5:1:1 H2O: H2O2: NH4OH.
  2. Place labware in the beaker and push the beaker to the back of the bench. 
  3. Let stand for 10 minutes.
  4. Dump the solution into the sink and rinse for 4 minutes.
  5. Fill the beakers with the solution of 5:1:1 H2O: :H2O2: HCl.
  6. Let stand for 10 minutes.
  7. Dump the solution into the sink and rinse for 4 minutes.
  8. Air dry on a clean wipe or use the  N2 blow gun to dry the labware.

 

 

For smaller personal use labware to be used at a contaminated wetbench use a clean pyrex (glass) or Nalgene beaker at wbgen or wbgaas:

  1. Fill the beaker with the solution of 5:1:1 H2O: H2O2: NH4OH.
  2. Place labware in the beaker and push the beaker to the back of the bench.  If you are cleaning a cassette mix the chemicals in the polypro box and place the cassette in it.
  3. Let stand for 10 minutes.
  4. Dump the solution into the sink and rinse for 4 minutes.
  5. Fill the beakers with the solution of 5:1:1 H2O: H2O2: HCl.
  6. Let stand for 10 minutes.
  7. Dump the solution into the sink and rinse for 4 minutes.
  8. Air dry on a clean wipe or use the  N2 blow gun to dry the labware.

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